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Microscope Resolution Equation:
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The microscope resolution is the smallest distance between two points that can still be distinguished as separate entities. It determines the level of detail that can be observed with a microscope.
The calculator uses the microscope resolution equation:
Where:
Explanation: The resolution is directly proportional to the wavelength of light used and inversely proportional to the numerical aperture of the objective lens.
Details: Understanding microscope resolution helps in selecting appropriate microscopy techniques and equipment for specific applications, ensuring that the required level of detail can be achieved.
Tips: Enter the wavelength of light in micrometers and the numerical aperture of the objective lens. Both values must be positive numbers.
Q1: What is the typical resolution of a light microscope?
A: For visible light (λ ≈ 0.5 μm) and high NA objective (NA ≈ 1.4), the resolution is about 0.2 μm.
Q2: How can I improve microscope resolution?
A: Use shorter wavelength light (e.g., blue instead of red), increase the numerical aperture, or use techniques like super-resolution microscopy.
Q3: What factors affect numerical aperture?
A: NA depends on the refractive index of the medium between the specimen and the lens, and the half-angle of the maximum cone of light that can enter the lens.
Q4: Does this equation apply to electron microscopes?
A: No, electron microscopes use different principles. Their resolution is determined by electron wavelength and aberrations in the electron optics.
Q5: What's the relationship between resolution and magnification?
A: Magnification makes details visible to the eye, but resolution determines whether those details can actually be distinguished. High magnification without sufficient resolution results in empty magnification.